Synergistic Binding of Nucleotides and Inhibitors to cAMP-dependent Protein Kinase Examined by Acrylodan Fluorescence Spectroscopy
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چکیده
منابع مشابه
THE EFFECT OF THEOPHYLLINE ON THE KINETICS OF cAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT, cAMP, PROTEIN KINASE INHIBITOR AND THEIR RELATIONSHIP IN LUNG TISSUE
We have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. The results show that the point of highest concentration of the C subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 Ilg/ InL and 10µg/mL theophylline, this is shifted to I.S an...
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Introduction cAMP and cGMP are second messengers that play important roles in intracellular signal transduction of various external stimuli. Major functions of both are the activation of cAMP-dependent protein kinase A (PKA) and cGMP-dependent protein kinase G (PKG), respectively. PKA and PKG are members of the serine-threonine protein kinase superfamily and are involved in the control of vario...
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متن کاملthe effect of theophylline on the kinetics of camp-dependent protein kinase catalytic subunit, camp, protein kinase inhibitor and their relationship in lung tissue
we have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. the results show that the point of highest concentration of the c subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 ilg/ inl and 10µg/ml theophylline, this is shifted to i.s and 2....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.3.1507